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How do you inject a sample in HPLC?
High-performance liquid chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify components in a sample. The success of an HPLC analysis largely depends on the proper injection of the sample into the system. Sample injection is a critical step that requires precision and attention to detail to achieve accurate and reproducible results. Injecting a sample in high-performance liquid chromatography (HPLC) involves several steps to ensure accurate and rep...
How Chromatography Empowers Drug Detection: A Powerful Analytical Technique
Chromatography, an indispensable analytical technique, plays a pivotal role in drug detection. It is a versatile method that allows scientists and forensic experts to separate and identify drug compounds from complex samples with high precision and accuracy. By employing various chromatographic techniques, such as gas chromatography (GC), liquid chromatography (LC), and thin-layer chromatography (TLC), drug detection becomes possible even in trace amounts. In this blog, we will delve into th...
Navigating Micro Inserts for HPLC Vials: A Comprehensive Guide
Choosing the right micro insert for High-Performance Liquid Chromatography (HPLC) vials is paramount for achieving precise and reproducible results in analytical workflows. Here’s a concise guide to help you make informed decisions: Sample Volume Consideration: Assess your sample volume requirements. Micro inserts come in various volumes, ensuring compatibility with a spectrum of sample sizes.Compatibility with Vial: Ensure the micro insert is compatible with the chosen HPLC vial. A...
Common faults and solutions of automatic samplers(1)
The main components of the autosampler are the injection needle, needle seat seal, injection valve and syringe. If maintenance is improper or not timely, the injection needle will easily bend and produce burrs, and the needle seat and injection valve will leak and become clogged, resulting in abnormalities such as ghost peaks, residual peak reproducibility, and insufficient quantitative analysis reproducibility. The syringe will produce bubbles, resulting in inaccurate injection. Therefore, ...
Ensuring Sample Integrity: Navigating EPA Storage Vials Stability Guidelines
In the field of environmental analysis, the reliability and accuracy of results hinge on the stability of stored samples. The U.S. Environmental Protection Agency (EPA) has established stringent guidelines to govern the storage of environmental samples in vials, emphasizing the preservation of sample integrity over time. Key Guidelines: Material Selection: EPA recommends the use of vials made from inert materials such as borosilicate glass or specially treated plastics to minimize samp...
Routine Maintenance of Autosampler
Autosampler failures can sometimes cause headaches. To find out the cause of the fault and effectively solve it, we must first start with daily maintenance and upkeep. 1. Clean the chromatography system in time After analyzing the sample and cleaning the chromatographic column, use a PEEK tube to connect it to the original position of the chromatographic column, clean it with degassed pure water at a flow rate of 1.5 mL/min for 1.5 hours, and then clean it with methanol under the same ...