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How do you select diluent in HPLC?
High-performance liquid chromatography (HPLC) is a powerful analytical technique used in various industries, including pharmaceuticals, environmental analysis, and food testing. One crucial aspect of HPLC sample preparation is selecting the appropriate diluent. The diluent plays a significant role in achieving accurate and reliable results by ensuring sample integrity, minimizing matrix effects, and optimizing chromatographic performance. In this article, we will explore the importance of se...
One of the “Three Key Points” for HPLC Instrument Operation: Filtration(1)
Any particulate matter entering the HPLC system will be blocked by the sieve plate at the inlet end of the column. The final result will be blockage of the column, which is characterized by increased system pressure and deformation of the chromatographic peaks. Therefore, various preventive measures should be taken, including operating procedures and various filtration designs of commercial instruments themselves, in an effort to prevent or reduce particulate matter from entering the HPLC sy...
One of the “Three Key Points” for HPLC Instrument Operation: Mobile Phase Degassing
Degassing the mobile phase is a very effective measure to avoid problems with the HPLC system and to obtain ideal data smoothly. It is undesirable to have air bubbles in the system. HPLC pumps must generate a lot of force when transporting liquids. Since the compression ratio of gases is much larger than that of liquids, when bubbles exist, you will observe an instantaneous decrease in flow rate and system pressure. If this bubble is large enough, the liquid phase pump will not be able ...
When using micro inserts in HPLC analysis, are there any special instructions or considerations?
Micro inserts are valuable accessories in HPLC analysis, designed to enhance the accuracy and efficiency of chromatographic experiments. These tiny devices play a crucial role in sample containment and injection precision, ensuring optimal performance and reliable results. However, to make the most of micro inserts in HPLC analysis, there are specific instructions and considerations that need to be kept in mind. Let’s delve into them! 1. Compatibility is Key: When selecting micro in...
Analysis and treatment methods of causes of chromatographic peak tailing(Ⅱ)
Last time we explained the causes of liquid chromatography column tailing. Today we will explain the causes of gas chromatography peak tailing.Generally speaking, when dealing with gas chromatography peak tailing problems, it can be summed up in one sentence: XXX sample is tailing on XXX chromatographic column. What is the reason? GC peak tailing 1. Active ingredient tailing Polar or reactive compounds are easily adsorbed by active sites in the sample flow path and appear tailing. T...
Static Headspace-gas chromatography Sample Introduction Method
Static headspace sampling of gas chromatography is to seal liquid or solid sample solution in a container (balance vial, headspace sample vial), and heat it at a certain temperature (equilibrium temperature) for a period of time (equilibrium time), so that the gas-liquid or The gas-solid two-phase reaches equilibrium, and then the gas phase is taken into a gas chromatograph for analysis. Static headspace sampling is also called balanced headspace sampling or a gas phase extraction sampling. ...