In this article, we will explore the importance of selecting the right diluent in HPLC and provide practical guidelines to help you make informed choices for your specific analytical needs.
Any particulate matter entering the HPLC system will be blocked by the sieve plate at the inlet end of the column. The final result will be blockage of the column, which is characterized by increased system pressure and deformation of the chromatographic peaks. Therefore, various preventive measures should be taken, including operating procedures and various filtration designs of commercial instruments themselves, in an effort to prevent or reduce particulate matter from entering the HPLC system, thereby extending the service life of the instrument and chromatographic column, and improving the reliability of data.
Degassing the mobile phase is a very effective measure to avoid problems with the HPLC system and to obtain ideal data smoothly. It is undesirable to have air bubbles in the system. HPLC pumps must generate a lot of force when transporting liquids.
Micro inserts are valuable accessories in HPLC analysis, designed to enhance the accuracy and efficiency of chromatographic experiments. These tiny devices play a crucial role in sample containment and injection precision, ensuring optimal performance and reliable results.
Last time we explained the causes of liquid chromatography column tailing. Today we will explain the causes of gas chromatography peak tailing.
Generally speaking, when dealing with gas chromatography peak tailing problems, it can be summed up in one sentence: XXX sample is tailing on XXX chromatographic column. What is the reason?
There are different sample introduction methods for gas chromatograph static headspace sampling, and you can choose the appropriate sampling method according to your own needs to reduce unnecessary troubles. Static headspace intrductions are available as syringe injections, equilibrium pressurized injections and dosing ring pressurized injections.
Choosing the right chromatography vial requires careful consideration of material, size, cap format, septum compatibility, instrument fit, and more. By matching your vial selection to your specific analytical needs, you can improve the accuracy and reproducibility of your chromatographic analysis, ultimately resulting in more robust and reliable results.
Headspace vials are available in different capacities and sizes and can be selected according to different situations. The selection of a suitable headspace vial volume can be determined according to the needs of gas chromatography analysis, column requirements and sample characteristics to ensure the accuracy and reliability of the experiment.
HPLC vials are used in the pharmaceutical and biopharmaceutical industry, food and beverage industry, oil and gas, environmental testing laboratories, and more. Rising demand for technologically advanced autosampler vials coupled with growing demand for improved sample safety and integrity has witnessed strong growth in the autosampler vials market.
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